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Improved solubility of replication factor C (RFC) Walker A mutants
Protein insolubility often poses a significant problem during purification protocols and in enzyme assays, especially for eukaryotic proteins expressed in a recombinant bacterial system. The limited solubility of replication factor C (RFC), the clamp loader complex from Saccharomyces cerevisiae, has...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3567619/ https://ncbi.nlm.nih.gov/pubmed/22469630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2012.03.010 |
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