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Complete Solubilization and Purification of Recombinant Human Growth Hormone Produced in Escherichia coli

High-level expression of recombinant human growth hormone (hGH) in Escherichia coli (E. coli) leads to the formation of insoluble aggregates as inclusion bodies devoid of biological activity. Until recently, significant efforts have been made to improve the recovery of active hGH from inclusion bodi...

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Bibliografische gegevens
Hoofdauteurs: Kim, Min-Ji, Park, Hyun Soo, Seo, Kyung Hye, Yang, Hyo-Jin, Kim, Sook-Kyung, Choi, Jun-Hyuk
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2013
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3567055/
https://ncbi.nlm.nih.gov/pubmed/23409149
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0056168
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