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Complete Solubilization and Purification of Recombinant Human Growth Hormone Produced in Escherichia coli

High-level expression of recombinant human growth hormone (hGH) in Escherichia coli (E. coli) leads to the formation of insoluble aggregates as inclusion bodies devoid of biological activity. Until recently, significant efforts have been made to improve the recovery of active hGH from inclusion bodi...

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Detalhes bibliográficos
Main Authors: Kim, Min-Ji, Park, Hyun Soo, Seo, Kyung Hye, Yang, Hyo-Jin, Kim, Sook-Kyung, Choi, Jun-Hyuk
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3567055/
https://ncbi.nlm.nih.gov/pubmed/23409149
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0056168
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