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An improved synthesis of a fluorophosphonate–polyethylene glycol–biotin probe and its use against competitive substrates

The fluorophosphonate (FP) moiety attached to a biotin tag is a prototype chemical probe used to quantitatively analyze and enrich active serine hydrolases in complex proteomes in an approach called activity-based protein profiling (ABPP). In this study we have designed a novel synthetic route to a...

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Hlavní autoři: Xu, Hao, Sabit, Hairat, Amidon, Gordon L, Showalter, H D Hollis
Médium: Artigo
Jazyk:Inglês
Vydáno: Beilstein-Institut 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3566859/
https://ncbi.nlm.nih.gov/pubmed/23400700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3762/bjoc.9.12
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