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An improved synthesis of a fluorophosphonate–polyethylene glycol–biotin probe and its use against competitive substrates
The fluorophosphonate (FP) moiety attached to a biotin tag is a prototype chemical probe used to quantitatively analyze and enrich active serine hydrolases in complex proteomes in an approach called activity-based protein profiling (ABPP). In this study we have designed a novel synthetic route to a...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Beilstein-Institut
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3566859/ https://ncbi.nlm.nih.gov/pubmed/23400700 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3762/bjoc.9.12 |
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