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Protein Kinase Activity from Vaccinia Virions: Solubilization and Separation into Heat-Labile and Heat-Stable Components

A protein kinase was solubilized from whole vaccinia virions by using a solution containing deoxycholate, dithiothreitol, and sodium or potassium chloride. The released enzyme was completely dependent on Mg(2+) and was greatly stimulated by added basic proteins such as protamine or histones. Dithiot...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:J Virol
Main Authors: Kleiman, Jay, Moss, Bernard
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology (ASM) 1973
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC356685/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4776967/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.12.4.684-689.1973
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