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Protein Kinase Activity from Vaccinia Virions: Solubilization and Separation into Heat-Labile and Heat-Stable Components

A protein kinase was solubilized from whole vaccinia virions by using a solution containing deoxycholate, dithiothreitol, and sodium or potassium chloride. The released enzyme was completely dependent on Mg(2+) and was greatly stimulated by added basic proteins such as protamine or histones. Dithiot...

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Bibliografische gegevens
Hoofdauteurs: Kleiman, Jay, Moss, Bernard
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1973
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC356685/
https://ncbi.nlm.nih.gov/pubmed/4776967
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