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High throughput, detailed, cell-specific neuroanatomy of dendritic spines using microinjection and confocal microscopy

Morphological features such as size, shape and density of dendritic spines have been shown to reflect important synaptic functional attributes and potential for plasticity. Here we describe in detail a protocol for obtaining detailed morphometric analysis of spines using microinjection of fluorescen...

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Detalhes bibliográficos
Main Authors: Dumitriu, Dani, Rodriguez, Alfredo, Morrison, John H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3566769/
https://ncbi.nlm.nih.gov/pubmed/21886104
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.389
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