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Studying calcium triggered vesicle fusion in a single vesicle-vesicle content/lipid mixing system

This Protocol describes a single vesicle-vesicle microscopy system to study Ca(2+)-triggered vesicle fusion. Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1. Acceptor vesicles contain reconstituted syntaxin and SNAP-25, and are tethered to a PEG-coated glass surface. Donor ves...

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Detaylı Bibliyografya
Asıl Yazarlar: Kyoung, Minjoung, Zhang, Yunxiang, Diao, Jiajie, Chu, Steven, Brunger, Axel T.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2012
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3566647/
https://ncbi.nlm.nih.gov/pubmed/23222454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.134
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