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Studying calcium triggered vesicle fusion in a single vesicle-vesicle content/lipid mixing system
This Protocol describes a single vesicle-vesicle microscopy system to study Ca(2+)-triggered vesicle fusion. Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1. Acceptor vesicles contain reconstituted syntaxin and SNAP-25, and are tethered to a PEG-coated glass surface. Donor ves...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3566647/ https://ncbi.nlm.nih.gov/pubmed/23222454 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.134 |
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