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Mapping Inhibitor Binding Modes on an Active Cysteine Protease via NMR Spectroscopy
Cruzain is a member of the papain/cathepsin-L family of cysteine proteases, and the major cysteine protease of the protozoan Trypanosoma cruzi, the causative agent of Chagas’ disease. We report an auto-induction methodology that provides soluble-cruzain at high yields (> 30 mg per liter in minima...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3566641/ https://ncbi.nlm.nih.gov/pubmed/23181936 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi301305k |
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