Loading...
Simple and inexpensive three-step rapid amplification of cDNA 5′ ends using 5′ phosphorylated primers
Rapid amplification of cDNA 5′ ends (5′-RACE) is routinely used for the sequence analysis of the upstream noncoding regions of cellular mRNAs; however, it represents a tedious and cost-intensive procedure. By employing 5′ phosphorylated gene-specific primers for first-strand cDNA synthesis, we cut s...
Na minha lista:
| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Academic Press
2013
|
| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3562438/ https://ncbi.nlm.nih.gov/pubmed/23123427 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.10.031 |
| Tags: |
Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!
|