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Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
Maturation of eukaryotic mRNAs involves 3' end formation, which involves the addition of a poly(A) tail. In order to map the 3' end of a gene, the traditional method of choice is 3' rapid amplification of cDNA ends (3' RACE). Protocols for 3' RACE require the careful design...
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| Pubblicato in: | J Vis Exp |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MyJove Corporation
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5933262/ https://ncbi.nlm.nih.gov/pubmed/29658915 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57318 |
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