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PUB-NChIP—“in vivo biotinylation” approach to study chromatin in proximity to a protein of interest
We have developed an approach termed PUB-NChIP (proximity utilizing biotinylation with native ChIP) to purify and study the protein composition of chromatin in proximity to a nuclear protein of interest. It is based on coexpression of (1) a protein of interest, fused with the bacterial biotin ligase...
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| Asıl Yazarlar: | , , , , , , , , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Cold Spring Harbor Laboratory Press
2013
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3561874/ https://ncbi.nlm.nih.gov/pubmed/23038767 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.134874.111 |
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