PUB-NChIP—“in vivo biotinylation” approach to study chromatin in proximity to a protein of interest

We have developed an approach termed PUB-NChIP (proximity utilizing biotinylation with native ChIP) to purify and study the protein composition of chromatin in proximity to a nuclear protein of interest. It is based on coexpression of (1) a protein of interest, fused with the bacterial biotin ligase...

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Bibliografiske detaljer
Main Authors: Shoaib, Muhammad, Kulyyassov, Arman, Robin, Chloé, Winczura, Kinga, Tarlykov, Pavel, Despas, Emmanuelle, Kannouche, Patricia, Ramanculov, Erlan, Lipinski, Marc, Ogryzko, Vasily
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2013
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3561874/
https://ncbi.nlm.nih.gov/pubmed/23038767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.134874.111
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