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Fluorescence-force spectroscopy maps two-dimensional reaction landscape of the Holliday junction

Despite the recent advances in single molecule manipulation techniques, purely mechanical approaches can not detect subtle conformational changes in the biologically important regime of weak forces. We developed a hybrid scheme combining force and fluorescence which allowed us to examine the effect...

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Autors principals: Hohng, Sungchul, Zhou, Ruobo, Nahas, Michelle K., Yu, Jin, Schulten, Klaus, Lilley, David M. J., Ha, Taekjip
Format: Artigo
Idioma:Inglês
Publicat: 2007
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3558530/
https://ncbi.nlm.nih.gov/pubmed/17932299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1146113
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