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Affinity Labeling of Hepatitis C Virus Replicase with a Nucleotide Analog: Identification of binding site
We have used an ATP analog, 5′-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA), to modify HCV replicase in order to identify ATP binding site in the enzyme. FSBA inactivates HCV replicase activity in a concentration dependent manner with a binding stoichiometry of 2 moles of FSBA per mole of enzyme. The...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3556826/ https://ncbi.nlm.nih.gov/pubmed/23268692 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi301098g |
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