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On Measuring miRNAs after Transient Transfection of Mimics or Antisense Inhibitors

The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To achieve this there is widespread use of both exogenous double-stranded miRNA mimics for transient over-expression, and single stranded antisense RNAs (antimiRs) for miRNA inhibition. The success of these manip...

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Autors principals: Thomson, Daniel W., Bracken, Cameron P., Szubert, Jan M., Goodall, Gregory J.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3554668/
https://ncbi.nlm.nih.gov/pubmed/23358900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0055214
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