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Transfection of Escherichia coli Spheroplasts IV. Transfection of rec(+) and rec(−) Spheroplasts by Native, Denatured, and Renatured T5 Bacteriophage DNA After Repair of Single-Strand Breaks by Polynucleotide Ligase

Transfection of Escherichia coli spheroplasts by native T5 phage DNA was not affected by treatment with polynucleotide ligase. Denatured T5 phage DNA infectivity, only 0.1% of the native DNA level, was increased slightly by polynucleotide ligase treatment. Renatured T5 phage DNA infectivity was also...

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Bibliographic Details
Main Authors: Benzinger, R., Scheible, P.
Format: Artigo
Language:Inglês
Published: 1974
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC355402/
https://ncbi.nlm.nih.gov/pubmed/4596299
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