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Transfection of Escherichia coli spheroplasts with a bacteriophage Mu DNA-protein complex.

We disrupted bacteriophage Mu particles by freeze-thaw treatment and recovered the DNA by CsCl density gradient centrifugation. This CsCl-purified DNA had a buoyant density which was indistinguishable from that of phenol-extracted Mu DNA. It was, however, 10(3) times more infective than phenol-extra...

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Autors principals: Chase, C D, Benzinger, R H
Format: Artigo
Idioma:Inglês
Publicat: 1982
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC256058/
https://ncbi.nlm.nih.gov/pubmed/6211551
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