Transfection of Escherichia coli spheroplasts with a bacteriophage Mu DNA-protein complex.
We disrupted bacteriophage Mu particles by freeze-thaw treatment and recovered the DNA by CsCl density gradient centrifugation. This CsCl-purified DNA had a buoyant density which was indistinguishable from that of phenol-extracted Mu DNA. It was, however, 10(3) times more infective than phenol-extra...
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| 發表在: | J Virol |
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| Principais autores: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1982
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC256058/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6211551/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.42.1.176-185.1982 |
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