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Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy P(BAD)-Controlled Gene Expression in Gram-Negative Bacteria
We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are d...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3553786/ https://ncbi.nlm.nih.gov/pubmed/23124231 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02926-12 |
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