Combinatorial Mutagenesis and Selection of Improved Signal Sequences and Their Application for High-Level Production of Translocated Heterologous Proteins in Escherichia coli

We previously designed the consensus signal peptide (CSP) and demonstrated that it can be used to strongly stimulate heterologous protein production in Escherichia coli. A comparative study using CSP and two bacterial signal sequences, pelB and ompA, showed that the effect of signal sequences on bot...

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מידע ביבליוגרפי
Main Authors: Heggeset, Tonje Marita Bjerkan, Kucharova, Veronika, Nærdal, Ingemar, Valla, Svein, Sletta, Håvard, Ellingsen, Trond Erling, Brautaset, Trygve
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2013
נושאים:
Biotechnology
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3553763/
https://ncbi.nlm.nih.gov/pubmed/23144128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02407-12
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