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Design and Optimization of Short DNA Sequences That Can Be Used as 5′ Fusion Partners for High-Level Expression of Heterologous Genes in Escherichia coli
The 5′ terminal nucleotide sequence of a gene is often a bottleneck in recombinant protein production. The ifn-α2b(S) gene is poorly expressed in Escherichia coli unless a translocation signal sequence (pelB) is fused to the 5′ end of the gene. A combined in silico and in vivo analysis reported here...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3811499/ https://ncbi.nlm.nih.gov/pubmed/23974137 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01676-13 |
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