Mouse TU tagging: a chemical/genetic intersectional method for purifying cell type-specific nascent RNA

Transcriptional profiling is a powerful approach for understanding development and disease. Current cell type-specific RNA purification methods have limitations, including cell dissociation trauma or inability to identify all RNA species. Here, we describe “mouse thiouracil (TU) tagging,” a genetic...

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Bibliografske podrobnosti
Main Authors: Gay, Leslie, Miller, Michael R., Ventura, P. Britten, Devasthali, Vidusha, Vue, Zer, Thompson, Heather L., Temple, Sally, Zong, Hui, Cleary, Michael D., Stankunas, Kryn, Doe, Chris Q.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2013
Teme:
Resource/Methodology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3553287/
https://ncbi.nlm.nih.gov/pubmed/23307870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.205278.112
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