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A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement
A significantly improved DNA polymerase fidelity assay, based on a gapped plasmid containing the lacZα reporter gene in a single-stranded region, is described. Nicking at two sites flanking lacZα, and removing the excised strand by thermocycling in the presence of complementary competitor DNA, is us...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Academic Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3552156/ https://ncbi.nlm.nih.gov/pubmed/23098700 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.10.019 |
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