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In-gel Nonspecific Proteolysis for Elucidating Glycoproteins (INPEG) – A Method for Targeted Protein-Specific Glycosylation Analysis in Complex Protein Mixtures

Determining protein-specific glycosylation in protein mixtures remains a difficult task. A common approach is to use gel electrophoresis to isolate the protein followed by glycan release from the identified band. However, gel bands are often composed of several proteins. Hence, release of glycans fr...

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Bibliografiske detaljer
Main Authors: Nwosu, Charles C., Huang, Jincui, Aldredge, Danielle L., Strum, John S., Hua, Serenus, Seipert, Richard R., Lebrilla, Carlito B.
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3547527/
https://ncbi.nlm.nih.gov/pubmed/23215446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac302574f
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