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Modification of residue 42 of the active site loop with a lysine-mimetic sidechain rescues isochorismate-pyruvate lyase activity in Pseudomonas aeruginosa PchB

PchB is an isochorismate-pyruvate lyase from Pseudomonas aeruginosa. A positively charged lysine residue is located in a flexible loop that behaves as a lid to the active site, and the lysine residue is required for efficient production of salicylate. A variant of PchB that lacks the lysine at resid...

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Detalhes bibliográficos
Main Authors: Olucha, José, Meneely, Kathleen M., Lamb, Audrey L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3546224/
https://ncbi.nlm.nih.gov/pubmed/22970849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300472n
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