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Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications

Many metabolic engineering and genetic engineering applications in yeast rely on the use of plasmids. Despite their pervasive use and the diverse collections available, there is a fundamental lack of understanding of how commonly used DNA plasmids affect the cell’s ability to grow and how the choice...

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Autori principali: Karim, Ashty S., Curran, Kathleen A., Alper, Hal S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3546148/
https://ncbi.nlm.nih.gov/pubmed/23107142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1567-1364.12016
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