Dialysis Purification of Integrase-DNA Complexes Provides High-Resolution Atomic Force Microscopy Images: Dimeric Recombinant HIV-1 Integrase Binding and Specific Looping on DNA

It remains difficult to obtain high-resolution atomic force microscopy images of HIV-1 integrase bound to DNA in a dimeric or tetrameric fashion. We therefore constructed specific target DNAs to assess HIV-1 integrase binding and purified the complex by dialysis prior to analysis. Our resulting atom...

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Main Authors: Tsuruyama, Tatsuaki, Nakai, Tonau, Ohmori, Rei, Ozeki, Munetaka, Tamaki, Keiji, Yoshikawa, Kenichi
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3544922/
https://ncbi.nlm.nih.gov/pubmed/23341952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0053572
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