Novel In Situ Pretreatment Method for Significantly Enhancing the Signal In MALDI-TOF MS of Formalin-Fixed Paraffin-Embedded Tissue Sections

The application of matrix-assisted laser desorption/ionization (MALDI)-based mass spectrometry (MS) to the proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue presents significant technical challenges. In situ enzymatic digestion is frequently used to unlock formalin-fixed tissues f...

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Bibliografski detalji
Glavni autori: Kakimoto, Yu, Tsuruyama, Tatsuaki, Yamamoto, Takushi, Furuta, Masaru, Kotani, Hirokazu, Ozeki, Munetaka, Yoshizawa, Akihiko, Haga, Hironori, Tamaki, Keiji
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2012
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416821/
https://ncbi.nlm.nih.gov/pubmed/22899997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0041607
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