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Optimization of Taq DNA polymerase enzyme expression in Escherichia coli
BACKGROUND: In the present study, we optimized the experimental conditions using pET15b expression vector to obtain large amounts of Taq DNA polymerase. MATERIALS AND METHODS: Correct framing of the gene in the expression vector pET15b and its orientation were analyzed by digestion and sequencing. P...
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| Auteurs principaux: | , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Medknow Publications & Media Pvt Ltd
2012
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3544109/ https://ncbi.nlm.nih.gov/pubmed/23326812 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4103/2277-9175.103004 |
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