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Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology
BACKGROUND: Expression of heterologous proteins at large scale is often a challenging job due to plasmid instability, accumulation of acetate and oxidative damage in bioreactors. Therefore, it is necessary to optimize parameters influencing cell growth and expression of recombinant protein. METHODS:...
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| Publicado no: | Avicenna J Med Biotechnol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Avicenna Research Institute
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6490408/ https://ncbi.nlm.nih.gov/pubmed/31057718 |
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