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In vitro culture of epicardial cells from adult zebrafish heart on a fibrin matrix

We describe here a protocol for culturing epicardial cells from adult zebrafish hearts, which have a unique regenerative capacity after injury. Briefly, zebrafish hearts first undergo ventricular amputation or sham operation. Next, the hearts are excised and explanted onto fibrin gels prepared in ad...

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Detalhes bibliográficos
Main Authors: Kim, Jieun, Rubin, Nicole, Huang, Ying, Tuan, Tai-Lan, Lien, Ching-Ling
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3541820/
https://ncbi.nlm.nih.gov/pubmed/22262006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.440
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