Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein.
Treatment of purified polyoma virions with 6 M guanidine-hydrochloride and 0.01 M beta-mercaptoethanol resulted in the immediate loss of both hemagglutinating and plaque-forming ability. Gel filtration through Sepharose CL-6B beads allowed separation of the dimer, VP1, VP2, VP3, and histone proteins...
Uloženo v:
| Vydáno v: | J Virol |
|---|---|
| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1978
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC354182/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/211269/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.27.2.436-442.1978 |
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