Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein.

Treatment of purified polyoma virions with 6 M guanidine-hydrochloride and 0.01 M beta-mercaptoethanol resulted in the immediate loss of both hemagglutinating and plaque-forming ability. Gel filtration through Sepharose CL-6B beads allowed separation of the dimer, VP1, VP2, VP3, and histone proteins...

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Main Authors: Brady, J N, Consigli, R A
Format: Artigo
Sprog:Inglês
Udgivet: 1978
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC354182/
https://ncbi.nlm.nih.gov/pubmed/211269
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