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RNAi in Cultured Mammalian Cells Using Synthetic siRNAs

RNA interference (RNAi) enables sequence-specific, experimentally induced silencing of almost any gene by tapping into innate regulatory mechanisms that are conserved among virtually all eukaryotes. In a typical RNAi experiment, an artificial silencing trigger directs the RNAi pathway toward a targe...

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Detalhes bibliográficos
Main Authors: Chang, Kenneth, Marran, Krista, Valentine, Amy, Hannon, Gregory J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3541682/
https://ncbi.nlm.nih.gov/pubmed/22949722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot071076
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