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An adhesion-based method for plasma membrane isolation: evaluating cholesterol extraction from cells and their membranes
A method to isolate large quantities of directly accessible plasma membrane from attached cells is presented. The method is based upon the adhesion of cells to an adsorbed layer of polylysine on glass plates, followed by hypotonic lysis with ice-cold distilled water and subsequent washing steps. Opt...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3541009/ https://ncbi.nlm.nih.gov/pubmed/19631189 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.07.027 |
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