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(77)Se Enrichment of Proteins Expands the Biological NMR Toolbox
Sulfur, a key contributor to biological reactivity, is not amendable to investigations by biological NMR spectroscopy. To utilize selenium as a surrogate, we have developed a generally applicable (77)Se isotopic enrichment method for heterologous proteins expressed in E. coli. We demonstrate (77)Se...
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| Main Authors: | , , , , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
2012
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3540199/ https://ncbi.nlm.nih.gov/pubmed/23159557 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.11.011 |
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