(77)Se Enrichment of Proteins Expands the Biological NMR Toolbox

Sulfur, a key contributor to biological reactivity, is not amendable to investigations by biological NMR spectroscopy. To utilize selenium as a surrogate, we have developed a generally applicable (77)Se isotopic enrichment method for heterologous proteins expressed in E. coli. We demonstrate (77)Se...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Schaefer, Stephanie A., Dong, Ming, Rubenstein, Renee P., Wilkie, Wayne A., Bahnson, Brian J., Thorpe, Colin, Rozovsky, Sharon
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2012
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3540199/
https://ncbi.nlm.nih.gov/pubmed/23159557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.11.011
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg