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(77)Se Enrichment of Proteins Expands the Biological NMR Toolbox

Sulfur, a key contributor to biological reactivity, is not amendable to investigations by biological NMR spectroscopy. To utilize selenium as a surrogate, we have developed a generally applicable (77)Se isotopic enrichment method for heterologous proteins expressed in E. coli. We demonstrate (77)Se...

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Detalhes bibliográficos
Main Authors: Schaefer, Stephanie A., Dong, Ming, Rubenstein, Renee P., Wilkie, Wayne A., Bahnson, Brian J., Thorpe, Colin, Rozovsky, Sharon
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3540199/
https://ncbi.nlm.nih.gov/pubmed/23159557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.11.011
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