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Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells

We show that the spectral phasor approach of the fluorescent dye Pyronin Y (PY) can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells. We applied spectral phasors to isolate intracellular RNA species with similar spectral properties. We identified at least 4...

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Bibliografische gegevens
Hoofdauteurs: Andrews, Laura M., Jones, Mark R., Digman, Michelle A., Gratton, Enrico
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Optical Society of America 2012
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3539199/
https://ncbi.nlm.nih.gov/pubmed/23304656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.4.000171
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