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Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells
We show that the spectral phasor approach of the fluorescent dye Pyronin Y (PY) can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells. We applied spectral phasors to isolate intracellular RNA species with similar spectral properties. We identified at least 4...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Optical Society of America
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3539199/ https://ncbi.nlm.nih.gov/pubmed/23304656 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.4.000171 |
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