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Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy

We demonstrate a methodology for tracing the clonal history of hematopoietic stem and progenitor cells (HSPCs) behavior in live tissues in 4 dimensions (4D). This integrates genetic combinatorial marking using lentiviral vectors encoding various fluorescent proteins (FPs) with advanced imaging metho...

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Bibliografiske detaljer
Main Authors: Malide, Daniela, Métais, Jean-Yves, Dunbar, Cynthia E.
Format: Artigo
Sprog:Inglês
Udgivet: American Society of Hematology 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3537316/
https://ncbi.nlm.nih.gov/pubmed/22995900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1182/blood-2012-06-440636
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