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Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.

A soluble RNA-dependent RNA polymerase was isolated from poliovirus-infected HeLa cells and was shown to copy poliovirus RNA in vitro. The enzyme was purified from a 200,000-X-g supernatant of a cytoplasmic extract of infected cells. The activity of the enzyme was measured throughout the purificatio...

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Detalhes bibliográficos
Publicado no:J Virol
Main Authors: Flanegan, J B, Van Dyke, T A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1979
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC353538/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/232168/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.32.1.155-161.1979
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