Limitations and possibilities of low cell number ChIP-seq

BACKGROUND: Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting material in the range of 1–20 million cells per immunoprecipitation,...

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Detalhes bibliográficos
Main Authors: Gilfillan, Gregor D, Hughes, Timothy, Sheng, Ying, Hjorthaug, Hanne S, Straub, Tobias, Gervin, Kristina, Harris, Jennifer R, Undlien, Dag E, Lyle, Robert
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3533509/
https://ncbi.nlm.nih.gov/pubmed/23171294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-13-645
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