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Digestion and depletion of abundant proteins improves proteomic coverage

Two major challenges in proteomics are the large number of proteins and their broad dynamic range within the cell. We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest and deplete abundant proteins with a method we call DigDeAPr. We validated the...

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Hlavní autoři: Fonslow, Bryan R., Stein, Benjamin D., Webb, Kristofor J., Xu, Tao, Choi, Jeong, Park, Sung Kyu, Yates, John R.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3531578/
https://ncbi.nlm.nih.gov/pubmed/23160281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2250
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