Spectrophotometric Assay for Amikacin Using Purified Kanamycin Acetyltransferase

A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme from R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl re...

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Bibliografiske detaljer
Udgivet i:Antimicrob Agents Chemother
Main Authors: Scarbrough, Emanual, Williams, Jeffrey W., Northrop, Dexter B.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1979
Fag:
Biosynthesis; Chemistry; Mechanisms of Action and Resistance
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC352826/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/485132/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.16.2.221
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