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Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
BACKGROUND: The XylS/Pm expression system has been used to produce recombinant proteins at industrial levels in Escherichia coli. Activation of transcription from the Pm promoter takes place in the presence of benzoic acid or derivatives of it. Previous mutagenesis studies resulted in identification...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2012
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3526546/ https://ncbi.nlm.nih.gov/pubmed/23031552 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-133 |
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