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The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA‐region corresponding to the 5′‐untranslated part of mRNA
Secondary structures and the short Shine–Dalgarno sequence in the 5′‐untranslated region of bacterial mRNAs (UTR) are known to affect gene expression at the level of translation. Here we report the use of random combinatorial DNA sequence libraries to study UTR function, applying the strong, σ(32)/σ...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Blackwell Publishing Ltd
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3815758/ https://ncbi.nlm.nih.gov/pubmed/21261932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2009.00107.x |
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