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Two-photon optogenetics of dendritic spines and neural circuits in 3D

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We apply the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual...

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Detalhes bibliográficos
Main Authors: Packer, Adam M., Peterka, Darcy S., Hirtz, Jan J., Prakash, Rohit, Deisseroth, Karl, Yuste, Rafael
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3518588/
https://ncbi.nlm.nih.gov/pubmed/23142873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2249
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