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Production of Recombineering Substrates With Standard-size PCR Primers
Recombineering is a powerful method for DNA manipulation. It has advantages over restriction endonuclease-based methods and is usually rapid. Typically recombineering uses long PCR primers (~65 bases), each of which contains a small region of target homology (~45 bases). We have developed a simple,...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2012
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3510332/ https://ncbi.nlm.nih.gov/pubmed/23003673 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1574-6968.12016 |
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